Resource collation from the 13th Crucifer Genetics Workshop 24th - 25th March 2002
Resources available for the Multinational Brassica Genome Project (MBGP)
Collated from discussion at Crucifer Genetics Workshop, March 2002.
| Resource |
Species |
Country |
Institution |
Contact |
Comments |
| RFLP markers |
Various |
USA |
Wisconsin |
Tom Osborn |
- Sequenced set due to be submitted to GenBank
|
| Various |
UK/Canada |
|
Derek Lydiate |
- probes sequenced but currently owned by two breeding companies. However, relative position in A.thaliana can be specified (Parkin et al., in press)
|
| SSR markers |
Various |
UK |
IACR/JIC |
Martin Trick |
- public domain set generated in BBSRC funded project (Martin Trick, Keith Edwards)
- Sequence of clones and primers in BrassicaDB
- Map data for 70 in B. napus in BrassicaDB
- SSRs currently being evaluated and mapped in B.oleracea and B. rapa at HRI
|
| Various |
Canada |
AAFC |
Andy Sharpe |
- SSRs from AAFC consortium – Consortium due to be polled first half 2002 whether able to release a subset into public domain
|
| Various |
|
Celera |
|
- Celera consortium SSRs – unknown status
|
| BAC libraries |
B. napus |
France |
INRA (Genoplante) |
Boulos Chaloub |
- from Genoplante consortium (separate unit for BAC libraries)
- 2x BAC libraries available (link)
|
| B. oleracea |
UK/USA |
HRI/TAMU |
Graham King |
- B. oleracea ‘BoB’ – publicly available from TAMU
- Being contiged as part of UK physical map programme
|
| B. oleracea |
UK |
PBL/JIC |
Ian Bancroft |
- B. oleracea ‘JBo’ - MTA
- Being contiged as part of UK physical map programme
|
| B. oleracea |
USA |
UC Davis |
Carlos Quiros |
- 18,000 clones (3x) (Carlos Quiros)
- var. italica ‘Early Big’
|
| B. oleracea |
USA |
U Georgia |
Andy Paterson |
- T01434 rapid cycling line 73,000 (10x)
- Same line as TIGR B. oleracea genomic sequencing
|
| B. oleracea |
USA |
U Georgia |
Andy Paterson |
- Badger Inbred 16 18,000 (3x)
|
Resources available for the Multinational Brassica Genome Project (MBGP)
Collated from discussion at Crucifer Genetics Workshop, March 2002
(cont)
| BAC libraries |
B. rapa |
UK |
PBL/JIC |
Ian Bancroft |
- B. rapa ‘JBr’ - MTA
- Being contiged as part of UK physical map programme
|
| B. rapa |
S. Korea |
Taejon |
Yong Pyo Lim |
|
| B. napus |
UK |
JIC |
Ian Bancroft |
- B. napus Tapidor large insert "JBnB"
- Partially contiged as part of EC – China collaboration
- B. napus Tapidor binary library "JBnY"
- Tapidor is a parent in "TN" population
|
| B. napus |
Canada |
AAFC |
Isobel Parkin |
- publicly available
- From DH parent of DH mapping population
|
| B. napus |
China |
|
|
- 80,000 clones 70kb inserts
- not public until about 2003
|
Resources available for the Multinational Brassica Genome Project (MBGP)
Collated from discussion at Crucifer Genetics Workshop, March 2002
(cont)
| BAC filter hybridisation |
|
USA |
U Georgia |
Andy Paterson |
- overgo probe sets being used for filter hybridisation
|
| ESTs |
B. napus |
France |
INRA (Genoplante) |
Michel Rennard |
- (+ GSTs)
- from Geniplante consortium
- target of 30,000
- will be available at least one year after obtained. Thus these will not be available until after April/May 2003.
- (Arabidopsis ESTs were available 6 months after made)
|
| |
B. napus |
Canada |
AAFC + |
Andy Sharpe
(Isobel Parkin) |
- website being set up which is searchable and will allow distribution on request subject to MTA
- Likely to be available in public domain in about 1 year (April 2003?)
|
| |
B. napus |
Germany |
GABI |
Friedt |
- cDNAs but industrial consortium, not in public domain
|
| BAC end sequences |
B. rapa |
S. Korea |
|
Yong Pyo Lim |
- From ‘Chifu’ library - BAC end sequences and cDNAs (to be put on arrays)
|
| Genomic sequences |
B. oleracea |
USA |
TIGR |
Chris Town |
- 300,000 in Genbank
- aiming for ...
|
Plant Resources
| Provisional list of Brassica genetic stocks available for deposit in Ohio Arabidopsis Stock Center (ARBC) c/o Randy Scholl, subject to further discussion and agreement with ABRC |
| Rapid cycling base populations |
|
USA |
Williams |
Rapid cycling stocks (CrGC) |
- One population for each of seven species
- must be maintained by cross pollination
|
| Mutant stocks |
|
USA |
Williams |
CrGC RCB mutants |
- 50 mutant or cytoplasm stocks, mostly B. rapa (Williams)
|
| |
USA |
Tom Osborn/
Rick Amasino |
EMS mutants |
- 50-200 B. oleracea EMS mutants, all SC (Osborn, Amasino)
|
| Mapping populations |
B. oleracea |
UK |
Graham King |
"AG" |
- A12DHd x GDDH33
- 120 DH lines, SC or semi-SI, (alboglabora x broc.)
- mapped DH lines available
|
| |
UK |
Mike Kearsey |
"AG" substitution lines |
- A12DHd x GDDH33
- mapped substitution lines available
|
| |
UK |
Graham King |
|
- parent lines of other mapping populations
|
| B. oleracea |
USA |
Tom Osborn |
"TB" population |
- 200 DH lines, SC, RC
- rapid cycling x broccoli
|
| B. rapa |
S. Korea |
Yong Pyo Lim |
"CK" population |
- 86 DH lines, SI (Chifu x ‘Kenshin’) (Lim)
- SI but being bulked
- 250 RI lines (i6-i7) to be released when ready
- Being anchored with public domain SSRs at HRI
|
| B. rapa |
USA |
Osborn |
"IR" population |
- 200 RI lines, SC
- (RC x Sarson)
|
| Mapping populations (cont.) |
B. rapa |
|
|
|
|
| B. napus |
USA |
Osborn |
"MS" pop. |
- 100 DH lines
- SC
- ‘Major’ x ‘Stella’
- (winter x spring)
|
| B. napus |
Canada |
Lydiate |
"SG" pop |
- 300 DH lines
- (spring x resyn.)
|
| B. napus |
France |
Rennard |
"DY" pop |
|
| B. napus |
China |
Meng |
"TN" pop |
- 200 DH lines
- (winter x semi winter)
- oilseed double low x high
|
| B. napus |
|
|
|
|
Download resource information as a Microsoft Word 97 file
|
